![]() Small-molecule displacement of a cryptic degron causes conditional protein degradation. Regulation of Cre recombinase by ligand-induced complementation of inactive fragments. Engineered allosteric activation of kinases in living cells. Activation of specific apoptotic caspases with an engineered small-molecule-activated protease. Dimeric ligands define a role for transcriptional activation domains in reinitiation. Chemical biology strategies for posttranslational control of protein function. Chemically controlled protein assembly: techniques and applications. Protein translocation as a tool: the current rapamycin story. Creating small-molecule-dependent switches to modulate biological functions. Specific modulation of protein activity by using a bioorthogonal reaction. Phthalimide conjugation as a strategy for in vivo target protein degradation. Small-molecule control of intracellular protein levels through modulation of the ubiquitin proteasome system. ![]() A chemical switch for inhibitor-sensitive alleles of any protein kinase. This developed methodology was applied to the conditional control of several cellular processes, including bioluminescence (luciferase), fluorescence (enhanced green fluorescent protein), protein translocation (nuclear localization sequence), DNA recombination (Cre) and gene editing (Cas9).īishop, A. Strategic placement of this group renders the protein inactive until deprotection through a bioorthogonal Staudinger reduction delivers the active wild-type protein. Genetic encoding of an ortho-azidobenzyloxycarbonyl amino acid using a pyrrolysyl transfer RNA synthetase/tRNA CUA pair in mammalian cells enables the site-specific introduction of a small-molecule-removable protecting group into the protein of interest. The approach uses a phosphine-mediated Staudinger reduction to activate protein function. ![]() Here we report a small-molecule switch that can be used to control protein activity. Using small molecules to control the function of proteins in live cells with complete specificity is highly desirable, but challenging. ![]()
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |